:Origin
Bacillus stearothermophilus HH

:Concentration
50000, u.a./ml

:Recognition site
GCG/C
C/GCG

:Reaction buffer
GM-buffer Y + BSA, (33mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1mM DTT + 100 μg/ml BSA.)

::Storage conditions
10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol.

:Storage temperature
-20°C

:Ligation
After 40-fold overdigestion with enzyme more than 90% of DNA fragments can be ligated and recut.

:Non-specific hydrolysis
No nonspecific activity was detected after incubation of 1 μg of DNA with 100 u.a. of enzyme for 16 hours at 65°C.

:Optimum temperature
50°C

:Inactivation 20 minutes
No

:Enzyme activity in % of maximum

B	G	O	W	Y
75-100	50-75	25-50	50-75	100

Product :
Cat. No.	Size
E143S	500 U
E143	2000 U
E144	10000 U