:Origin
E.coli strain that carries the cloned Hind II gene from Haemophilus influenzae

:Concentration
10000, u.a./ml

:Recognition site
GTY/RAC
CAR/YTG

:Reaction buffer
GM-buffer G + BSA, (10mM Tris-HCl (pH 7.6 at 25℃); 10 mM MgCl2; 50 mM NaCl; 1mM DTT + 100 μg/ml BSA.)

:Storage conditions
10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1mM EDTA; 1 mM DTT; 200 µg/ml BSA; 50% glycerol.

:Storage temperature
-20°C

:Ligation
After 10-fold overdigestion with enzyme 60% of DNA fragments can be ligated and recut. In the presence of 10% PEG ligation is better.

:Non-specific hydrolysis
No nonspecific activity was detected after incubation of 1 μg of DNA with 20 u.a. of enzyme for 16 hours at 37°C.

:Optimum temperature
37°C

:Inactivation 20 minutes
under 65°C

:Enzyme activity in % of maximum

B	G	O	W	Y
75-100	100	25-50	25-50	75-100

Product :
Cat. No.	Qty.
E201S	200 U
E201	1000 U
E202	5000 U