:Origin
E.coli strain that carries the cloned Hpa I gene from Haemophilus parainfluenzae

:Concentration
5000, u.a./ml

:Recognition site
GTT/AAC
CAA/TTG

:Reaction buffer
GM-buffer Y, (33mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1mM DTT.)

:Storage conditions
10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1mM EDTA; 1 mM DTT; 200 µg/ml BSA; 50% glycerol.

:Storage temperature
-20°C

:Ligation
After 5-fold overdigestion with enzyme about 60% of DNA fragments can be ligated and recut.

:Non-specific hydrolysis
No nonspecific activity was detected after incubation of 1 μg of DNA with 5 u.a. of enzyme for 16 hours at 37°C.

:Optimum temperature
37°C

:Inactivation 20 minutes
under 65°C

:Enzyme activity in % of maximum

B	G	O	W	Y
0-10	50-75	10-25	25-50	100

Product：
Cat. No.	Size
E077S	200 U
E077	500 U
E078	2500 U