:Origin
E.coli strain that carries the cloned Hpa II gene from Haemophilus parainfluenzae

:Concentration
10000, u.a./ml

:Recognition site
C/CGG
GGC/C

:Reaction buffer
GM-buffer B, (10mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1mM DTT.)

:Storage conditions
10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1mM EDTA; 1 mM DTT; 100 µg/ml BSA; 50% glycerol.

:Storage temperature
-20°C

:Ligation
After 10-fold overdigestion with enzyme more than 95% of DNA fragments can be ligated and recut.

:Non-specific hydrolysis
No nonspecific activity was detected after incubation of 1 μg of DNA with 10 u.a. of enzyme for 16 hours at 37°C.

:Optimum temperature
37°C

:Inactivation 20 minutes
under 80°C

:Enzyme activity in % of maximum

B	G	O	W	Y
100	50-75	10-25	25-50	50-75

Product：
Cat. No.	Size
E161S	400 U
E161	2000 U
E162	10000 U