Origin:
E.coli strain that carries the cloned Mnl I gene from Moraxella nonliquefaciens
Concentration:
10000, u.a./ml
Recognition site:
CCTC(N)7/
GGAG(N)6/
Reaction buffer:
GM-buffer G + BSA, (10mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT + 100 μg/ml BSA)
Storage conditions:
10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1mM EDTA; 1 mM DTT; 200 µg/ml BSA; 50% glycerol.
Storage temperature:
-20°C
Ligation:
After 10-fold overdigestion with enzyme 50% of DNA fragments can be ligated and recut.
Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Optimum temperature:
37°C
Inactivation 20 minutes:
under 65°C
Enzyme activity in % of maximum:
B | G | O | W | Y |
75-100 | 100 | 25-50 | 25-50 | 75-100 |
Product: | |
Cat. No. | Size |
E481S | 100 U |
E481 | 250 U |
E482 | 1250 U |