Origin:
E.coli strain that carries the cloned Hind III gene from Haemophilus influenzae Rd

Concentration:
20000, u.a./ml

Recognition site:
A/AGCTT
TTCGA/A

Reaction buffer:
GM-buffer W + BSA, (10mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl₂; 100 mM NaCl; 1mM DTT + 100 μg/ml BSA.)

Storage conditions:
10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol.

Storage temperature:
-20°C

Ligation:
After 50-fold overdigestion with enzyme 95% of DNA fragments can be ligated and recut.

Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 40 u.a. of enzyme for 16 hours at 37°C.

Optimum temperature:
37°C

Inactivation 20 minutes:
under 80°C

Enzyme activity in % of maximum:

B	G	O	W	Y
10-25	25-50	0-10	100	0-10

Product：
Cat. No.	Size
E073S	2500 U
E073	5000 U
E074	25000 U