Origin:
Actinobacillus suis CA

Concentration:
20000 – 50000, u.a./ml

Recognition site:
CC/SGG
GGS/CC

Reaction buffer:
GM-buffer Y, (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)

Storage conditions:
10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol.

Storage temperature:
-20°C

Ligation:
After 5-fold overdigestion with enzyme less than 10% of DNA fragments can be ligated.

Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 50 u.a. of enzyme for 16 hours at 37°C.

Optimum temperature:
37°C

Inactivation 20 minutes:
under 65°C

Enzyme activity in % of maximum:

B	G	O	W	Y
75-100	50-75	10-25	25-50	100

Product :
Cat. No.	Size
E257S	400 U
E257	2000 U
E258	10000 U