Origin:
Actinobacillus suis CA
Concentration:
20000 – 50000, u.a./ml
Recognition site:
CC/SGG
GGS/CC
Reaction buffer:
GM-buffer Y, (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Storage conditions:
10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol.
Storage temperature:
-20°C
Ligation:
After 5-fold overdigestion with enzyme less than 10% of DNA fragments can be ligated.
Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 50 u.a. of enzyme for 16 hours at 37°C.
Optimum temperature:
37°C
Inactivation 20 minutes:
under 65°C
Enzyme activity in % of maximum:
B | G | O | W | Y |
75-100 | 50-75 | 10-25 | 25-50 | 100 |
Product : | |
Cat. No. | Size |
E257S | 400 U |
E257 | 2000 U |
E258 | 10000 U |