Origin:
Bacillus species 19

Concentration:
10000 – 20000, u.a./ml

Recognition site:
C/CATGG
GGTAC/C

Reaction buffer:
GM-buffer 2W + BSA, (20 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl₂; 200 mM NaCl; 1 mM DTT + 100 μg/ml BSA.)

Storage conditions:
10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol.

Storage temperature:
-20°C

Ligation:
After 10-fold overdigestion with enzyme more than 90% of DNA fragments can be ligated and recut.

Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 20 u.a. of enzyme for 16 hours at 37°C.

Optimum temperature:
37°C

Inactivation 20 minutes:
under 65°C

Enzyme activity in % of maximum:

B	G	O	W	Y
0-10	10-25	50-75	75-100	10-25

Product：
Cat. No.	Size
E047S	200 U
E047	500 U
E048	2500 U