Origin:
Streptomyces fradiae 274
Concentration:
10000 – 30000, u.a./ml
Recognition site:
C/TCGAG
GAGCT/C
Reaction buffer:
GM-buffer B, (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.)
Storage conditions:
10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol.
Storage temperature:
-20°C
Ligation:
After 20-fold overdigestion with enzyme more than 90% of DNA fragments can be ligated and recut.
Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 20 u.a. of enzyme for 16 hours at 50°C.
Optimum temperature:
50°C
Inactivation 20 minutes:
under 65°C
Enzyme activity in % of maximum:
B | G | O | W | Y |
100 | 75-100 | 50-75 | 50-75 | 75-100 |
Product: | |
Cat. No. | Size |
E125S | 1000 U |
E125 | 5000 U |
E126 | 25000 U |