Origin:
E.coli strain that carries the cloned Xma I gene from Xanthomonas malvacearum

Concentration:
3000, u.a./ml

Recognition site:
C/CCGGG
GGGCC/C

Reaction buffer:
GM-buffer Y, (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 10 mM potassium acetate; 1 mM DTT.)

Storage conditions:
20 mM Tris-HCl (pH 7.6); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 µg/ml BSA; 50% glycerol.

Storage temperature:
-20°C

Ligation:
After 3-fold overdigestion with enzyme 95% of DNA fragments can be ligated. Of these 90% can be recut.

Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 3 u.a. of enzyme for 16 hours at 37°C.

Optimum temperature:
37°C

Inactivation 20 minutes:
under 65°C

Enzyme activity in % of maximum:

B	G	O	W	Y
75-100	50-75	0-10	0-10	100

Product：
Cat. No.	Size
E233S	100 U
E233	300 U
E234	1500 U