Origin:
E.coli strain that carries the cloned Tsp509 I gene from Sporosarcina species

Concentration:
5000, u.a./ml

Recognition site:
/AATT
TTAA/

Reaction buffer:
GM-buffer B + BSA, (10 mM Tris-HCl(pH 7.6 at 25°C); 10 mM MgCl₂;1 mM DTT + 100 μg/ml BSA.)

Storage conditions:
10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 µg/ml BSA; 50% glycerol.

Storage temperature:
-20°C

Ligation:
After 5-fold overdigestion with enzyme more than 95% of DNA fragments can be ligated and recut.

Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 5 u.a. of enzyme for 16 hours at 55℃.

Optimum temperature:
55°C

Inactivation 20 minutes:
under 65°C

Enzyme activity in % of maximum:

B	G	O	W	Y
100	75-100	50-75	50-75	75-100

Product：
Cat. No.	Size
E217S	200 U
E217	500 U
E218	2500 U