Origin:
Pseudomonas species N4

Concentration:
10000 – 30000, u.a./ml

Recognition site:
GGN/NCC
CCN/NGG

Reaction buffer:
GM-buffer Y, (33 mM Tris-acetate (pH 7.9 at 25℃); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)

Storage conditions:
10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol.

Storage temperature:
-20°C

Ligation:
After 10-fold overdigestion with enzyme more than 90% of DNA fragments can be ligated and recut.

Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 30 u.a. of enzyme for 16 hours at 37°C.

Optimum temperature:
37°C

Inactivation 20 minutes:
under 65°C

Enzyme activity in % of maximum:

B	G	O	W	Y
10-25	10-25	10-25	25-50	100

Product :
Cat. No.	Size
E089S	200 U
E089	1000 U
E090	5000 U