Origin:
Pseudomonas species 124B
Concentration:
10000 – 30000, u.a./ml
Recognition site:
GAGCT/C
C/TCGAG
Reaction buffer:
GM-buffer G, (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.)
Storage conditions:
10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 µg/ml BSA; 50% glycerol.
Storage temperature:
-20°C
Ligation:
After 20-fold overdigestion with enzyme more than 90% of DNA fragments can be ligated and recut.
Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 20 u.a. of enzyme for 16 hours at 37°C.
Optimum temperature:
37°C
Inactivation 20 minutes:
under 80°C
Enzyme activity in % of maximum:
B | G | O | W | Y |
75-100 | 100 | 10-25 | 0-10 | 75-100 |
Product : | |
Cat. No. | Size |
E107S | 500U |
E107 | 2000 U |
E108 | 10000 U |