Origin:
Zoogloea species 2

Concentration:
5000 – 20000, u.a./ml

Recognition site:
ATGCA/T
T/ACGTA

Reaction buffer:
GM-buffer B + BSA, (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl₂; 1 mM DTT + 100 μg/ml BSA.)

Storage conditions:
10 mM Tris-HCl (pH 7.5); 200 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol.

Storage temperature:
-20°C

Ligation:
After 10-fold overdigestion with enzyme more than 90% of DNA fragments can be ligated and recut.

Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 20 u.a. of enzyme for 16 hours at 37°C.

Optimum temperature:
37°C

Inactivation 20 minutes:
under 65°C

Enzyme activity in % of maximum:

B	G	O	W	Y
100	50-75	25-50	25-50	25-50

Product：
Cat. No.	Size
E145S	200 U
E145	1000 U
E146	5000 U