Origin:
Pseudomonas species C
Concentration:
10000 – 30000, u.a./ml
Recognition site:
CAC/GTG
GTG/CAC
Reaction buffer:
GM-buffer B + BSA, (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT + 100 μg/ml BSA.)
Storage conditions:
10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 10 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol.
Storage temperature:
-20°C; For longterm storage (>30 days), store at -70°C.
Ligation:
After 10-fold overdigestion with
enzyme more than 90% of DNA fragments can be ligated and recut.
Ligation is better in presence of 10% PEG.
Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 40 u.a. of enzyme for 16 hours at 37°C.
Optimum temperature:
37°C
Inactivation 20 minutes:
under 65°C
Enzyme activity in % of maximum:
B | G | O | W | Y |
100 | 50-75 | 0-10 | 0-10 | 50-75 |
Product: | |
Cat. No. | Size |
E475S | 400 U |
E475 | 2000 U |
E476 | 10000 U |