Psp0M I (Apa I^, Bsp120 I)

Origin:
E.coli strain that carries the cloned PspOM I gene from Pseudomonas species OM2164

Concentration:
10000, u.a./ml

Recognition site:
G/GGCCC
CCCGG/G

Reaction buffer:
GM-buffer Y, (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)

Storage conditions:
20 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 µg/ml BSA; 50% glycerol.

Storage temperature:
-20°C

Ligation:
After 10-fold overdigestion with enzyme more than 95% of DNA fragments can be ligated and recut.

Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 20 u.a. of enzyme for 16 hours at 37°C.

Optimum temperature:
37°C

Inactivation 20 minutes:
under 65°C

Enzyme activity in % of maximum:

BGOWY
75-10010-250-100-10100
Product:
Cat. No.Size
E215S400 U
E2151000 U
E2165000 U