Origin:
E.coli strain that carries the cloned PspOM I gene from Pseudomonas species OM2164
Concentration:
10000, u.a./ml
Recognition site:
G/GGCCC
CCCGG/G
Reaction buffer:
GM-buffer Y, (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Storage conditions:
20 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 µg/ml BSA; 50% glycerol.
Storage temperature:
-20°C
Ligation:
After 10-fold overdigestion with enzyme more than 95% of DNA fragments can be ligated and recut.
Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 20 u.a. of enzyme for 16 hours at 37°C.
Optimum temperature:
37°C
Inactivation 20 minutes:
under 65°C
Enzyme activity in % of maximum:
B | G | O | W | Y |
75-100 | 10-25 | 0-10 | 0-10 | 100 |
Product: | |
Cat. No. | Size |
E215S | 400 U |
E215 | 1000 U |
E216 | 5000 U |