Origin:
E.coli strain that carries the cloned Sfa N I gene from Streptococcus faecalis N

Concentration:
10000, u.a./ml

Recognition site:
GCATC(N)₅/
CGTAG(N)₉/

Reaction buffer:
GM-buffer O, (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl₂; 100 mM NaCl; 1 mM DTT.)

Storage conditions:
10 mM Tris-HCl (pH 7.5); 300 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 µg/ml BSA; 50% glycerol.

Storage temperature:
-20°C

Ligation:
After 10-fold overdigestion more than 95% of DNA fragments can be ligated and recut.

Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 20 u.a. of enzyme for 16 hours at 37°C.

Optimum temperature:
37°C

Inactivation 20 minutes:
under 80°C

Enzyme activity in % of maximum:

B	G	Y	W	Y
10-25	25-50	100	75-100	0-10

Product：
Cat. No.	Size
E165	100 U
E166	500 U