Origin:
E.coli strain that carries the cloned Sfi I gene from Streptomyces fimbriatus

Concentration:
10000, u.a./ml

Recognition site:
GGCCNNNN/NGGCC
CCGGN/NNNNCCGG

Reaction buffer:
GM-buffer G + BSA, (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl₂; 50 mM NaCl; 1 mM DTT + 100μg/ml BSA .)

Storage conditions:
10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol.

Storage temperature:
-20°C

Ligation:
After 10-fold overdigestion with enzyme 70% of DNA fragments can be ligated and recut. In the presence of 10% PEG ligation is better.

Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 20 u.a. of enzyme for 16 hours at 50°C.

Optimum temperature:
50°C

Inactivation 20 minutes:
under 65°C

Enzyme activity in % of maximum:

B	G	O	W	Y
75-100	100	25-50	25-50	25-50

Product：
Cat. No.	Size
E123	500 U
E124	2500 U