Origin:
Enterobacter gergoviae

Concentration:
5000 – 10000, u.a./ml

Recognition site:
GGC/GCC
CCG/CGG

Reaction buffer:
GM-buffer B + BSA, (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl₂; 1 mM DTT + 100 μg/ml BSA .)

Storage conditions:
10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol.

Storage temperature:
-20°C

Ligation:
After 5-fold overdigestion with enzyme 70% of DNA fragments can be ligated and recut.

Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 10 u.a. of enzyme for 16 hours at 37°C.

Optimum temperature:
37°C

Inactivation 20 minutes:
under 65°C

Enzyme activity in % of maximum:

B	G	O	W	Y
100	75-100	10-25	50-75	75-100

Product：
Cat. No.	Size
E243	200 U
E244	1000 U