Origin:
E.coli strain that carries the cloned Sma I gene from Serratia marcescens
Concentration:
20000, u.a./ml
Recognition site:
CCC/GGG
GGG/CCC
Reaction buffer:
GM-buffer Y, (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Storage conditions:
10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol.
Storage temperature:
-20°C
Ligation:
After 20-fold overdigestion with
enzyme more than 90% of DNA fragments can be ligated ( by using of high
concentration T4 DNA Ligase and 10% PEG )and recut.
Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 40 u.a. of enzyme for 16 hours at 25°C.
Optimum temperature:
25°C
Inactivation 20 minutes:
under 65°C
Enzyme activity in % of maximum:
| B | G | O | W | Y |
| 0-10 | 0-10 | 0-10 | 0-10 | 100 |
| Product： | |
| Cat. No. | Size |
| E177S | 500 U |
| E177 | 2000 U |
| E178 | 10000 U |