Origin:
Planococcus citreus 55

Concentration:
10000 – 20000, u.a./ml

Recognition site:
AGG/CCT
TCC/GGA

Reaction buffer:
GM-buffer Y, (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)

Storage conditions:
10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 µg/ml BSA; 50% glycerol.

Storage temperature:
-20°C

Ligation:
After 10-fold overdigestion with enzyme 70% of DNA fragments can be ligated and recut.

Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 20 u.a. of enzyme for 16 hours at 50°C.

Optimum temperature:
50°C

Inactivation 20 minutes:
under 80°C

Enzyme activity in % of maximum:

B	G	O	W	Y
75-100	75-100	50-75	25-50	100

Product：
Cat. No.	Size
E105S	500 U
E105	2000 U
E106	10000 U