Origin:
Pseudomonas species 6

Concentration:
1000 – 3000, u.a./ml

Recognition site
/CCWGG
GGWCC/

Reaction buffer:
GM-buffer B, (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl₂; 1 mM DTT.)

Storage conditions:
10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol.

Storage temperature:
-20°C

Ligation:
After 3-fold overdigestion with enzyme more than 95% of DNA fragments can be ligated and recut.

Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 6 u.a. of enzyme for 16 hours at 37°C.

Optimum temperature:
55°C

Inactivation 20 minutes:
under 80°C

Enzyme activity in % of maximum:

B	G	O	W	Y
100	50-75	10-25	25-50	75-100

Product：
Cat. No.	Size
E453	100 U
E454	500 U