Swa I ( Smi I )

Origin:
Streptococcus milleri S

Concentration:
10000 – 30000, u.a./ml

Recognition site:
ATTT/AAAT
TAAA/TTTA

Reaction buffer:
GM-buffer O + BSA, (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT + 100 μg/ml BSA.)

Storage conditions:
10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 µg/ml BSA; 50% glycerol.

Storage temperature:
-20°C

Ligation:
After 5-fold overdigestion with enzyme 80% of DNA fragments can be ligated and recut. Ligation >95% in presence of 10% PEG.

Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 30 u.a. of enzyme for 16 hours at 37°C.

Optimum temperature:
37°C

Inactivation 20 minutes:
under 65°C

Enzyme activity in % of maximum:

BGOWY
25-5025-5010075-10025-50
Product :
Cat. No.Qty.
E225S200 U
E2251000 U
E2265000 U