:Origin
Bacillus stearothermophilus AF

:Concentration
5000 – 20000, u.a./ml

:Recognition site
C/TTAAG
GAATT/C

:Reaction buffer
GM-buffer W + BSA, (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl₂; 100 mM NaCl; 1 mM DTT.)

:Storage conditions
10 mM Tris-HCl (pH 7.5); 200 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol.

:Storage conditions
10 mM Tris-HCl (pH 7.5); 200 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol.

Storage temperature: -20°C

:Ligation
After 5-fold overdigestion with enzyme more than 40% of the DNA fragments can be ligated and recut.

:Non-specific hydrolysis
No nonspecific activity was detected after incubation of 1 μg of DNA with 40 u.a. of enzyme for 16 hours at 55°C.

Optimum temperature: 55°C

Inactivation 20 minutes: under 80°C

:Enzyme activity in % of maximum

Product
Cat. No.	Size
E135	500 U
E136	2500 U

B	G	O	W	Y
10-25	25-50	75-100	100	25-50