:Origin
Bacillus stearothermophilus AF
:Concentration
5000 – 20000, u.a./ml
:Recognition site
C/TTAAG
GAATT/C
:Reaction buffer
GM-buffer W + BSA, (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)
:Storage conditions
10 mM Tris-HCl (pH 7.5); 200 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol.
:Storage conditions
10 mM Tris-HCl (pH 7.5); 200 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol.
Storage temperature: -20°C
:Ligation
After 5-fold overdigestion with enzyme more than 40% of the DNA fragments can be ligated and recut.
:Non-specific hydrolysis
No nonspecific activity was detected after incubation of 1 μg of DNA with 40 u.a. of enzyme for 16 hours at 55°C.
Optimum temperature: 55°C
Inactivation 20 minutes: under 80°C
:Enzyme activity in % of maximum
Product | |
Cat. No. | Size |
E135 | 500 U |
E136 | 2500 U |
B | G | O | W | Y |
10-25 | 25-50 | 75-100 | 100 | 25-50 |