Origin:
Arthrobacter citreus

Concentration:
10000-20000, u.a./ml

Recognition site:
R/AATTY
YTTAA/R

Reaction buffer:
GM-buffer W + BSA,(10 mM Tris-HC l(pH 8.5 at 25°C); 10 mM MgCl₂; 100 mM NaCl; 1 mM DTT + 100 μg/ml BSA.)

Storage conditions:
20 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 10 mM 2-mercaptoethanol; 50% glycerol

Storage temperature: -20°C

Ligation:
After 20-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.

Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 50 u.a. of enzyme for 16 hours at 50℃.

Optimum temperature: 50°C

Inactivation 20 minutes: under 80°C

Enzyme activity in % of maximum:

B	G	O	W	Y
25-50	50-75	50-75	100	10-25

Product
Cat. No.	Size
E013S	100 U
E013	500 U
E014	2500 U