Origin: Sphingobacterium mizutae M Concentration: 5000 – 10000, u.a./ml Recognition site: CAYNN/NNRTG GTRNN/NNYAC Reaction buffer: GM-buffer W, (10mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) Storage conditions: 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol. Storage temperature: […]
Origin: Moraxella species Concentration: 10000 – 20000, u.a./ml Recognition site: C/CGG GGC/C Reaction buffer: GM-buffer B, (10mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.) Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol. Storage temperature: -20°C Ligation: After 10-fold […]
Origin: Moraxella species A1 Concentration: 2000 – 10000, u.a./ml Recognition site: CMG/CKG GKC/GMC Reaction buffer: GM-buffer Y, (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) Storage conditions: 20 mM Tris-HCl (pH 7.6); 300 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 10 mM MgCl2; 200 […]