Origin:
Moraxella species A1
Concentration:
2000 – 10000, u.a./ml
Recognition site:
CMG/CKG
GKC/GMC
Reaction buffer:
GM-buffer Y, (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Storage conditions:
20 mM Tris-HCl (pH 7.6); 300 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 10 mM MgCl2; 200 µg/ml BSA; 50% glycerol.
Storage temperature:
-20°C; For long term stroage ( > 30 days), store at -70°C.
Ligation:
After 5-fold overdigestion with enzyme
60% of DNA fragments can be ligated and recut. In presence of 10% PEG
logation is better.
Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Optimum temperature:
37°C
Inactivation 20 minutes:
under 65°C
Enzyme activity in % of maximum:
B | G | O | W | Y |
10-25 | 75-100 | 10-25 | 25-50 | 100 |
Product: | |
Cat. No. | Size |
E191S | 100 U |
E191 | 500 U |
E192 | 2500 U |