Origin: Streptomyces fradiae 274 Concentration: 10000 – 30000, u.a./ml Recognition site: C/TCGAG GAGCT/C Reaction buffer: GM-buffer B, (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.) Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol. Storage temperature: -20°C Ligation: […]
Origin: E.coli strain that carries the cloned Xma I gene from Xanthomonas malvacearum Concentration: 3000, u.a./ml Recognition site: C/CCGGG GGGCC/C Reaction buffer: GM-buffer Y, (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 10 mM potassium acetate; 1 mM DTT.) Storage conditions: 20 mM Tris-HCl (pH 7.6); 50 mM NaCl; 0.1 mM EDTA; 1 […]
Origin: Micrococcus roseus X Concentration: 5000 – 15000, u.a./ml Recognition site: GAANN/NNTTC CTTNN/NNAAG Reaction buffer: GM-buffer W, (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) Storage conditions: 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol. Storage temperature: […]