Origin:
Micrococcus roseus X
Concentration:
5000 – 15000, u.a./ml
Recognition site:
GAANN/NNTTC
CTTNN/NNAAG
Reaction buffer:
GM-buffer W, (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)
Storage conditions:
10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol.
Storage temperature:
-20°C
Ligation:
After 5-fold overdigestion with enzyme more than 50% of DNA fragments can be ligated and recut.
Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Optimum temperature:
37°C
Inactivation 20 minutes:
under 65°C
Enzyme activity in % of maximum:
B | G | O | W | Y |
50-75 | 50-75 | 50-75 | 100 | 25-50 |
Product: | |
Cat. No. | Size |
E249S | 100 U |
E249 | 500 U |
E250 | 2500 U |