Origin: Actinobacillus suis CA Concentration: 20000 – 50000, u.a./ml Recognition site: CC/SGG GGS/CC Reaction buffer: GM-buffer Y, (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) Storage conditions: 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol. Storage temperature: […]
Origin: Micrococcus lylae 113 Concentration: 3000 – 5000, u.a./ml Recognition site: GG/CGCC CCGC/GG Reaction buffer: GM-buffer B, (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.) Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol. Storage temperature: -20°C Ligation: […]
Origin: Nocardia rubra Concentration: 5000 – 10000, u.a./ml Recognition site: TCG/CGA AGC/GCT Reaction buffer: GM-buffer W, (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) Storage conditions: 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol. Storage temperature: -20°C Ligation: After […]