Origin:
Nocardia rubra
Concentration:
5000 – 10000, u.a./ml
Recognition site:
TCG/CGA
AGC/GCT
Reaction buffer:
GM-buffer W, (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)
Storage conditions:
10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol.
Storage temperature:
-20°C
Ligation:
After 5-fold overdigestion with enzyme
about 20% of DNA fragments can be ligated. In the presence of 10% PEG
ligation is better.
Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Optimum temperature:
37°C
Inactivation 20 minutes:
under 80°C
Enzyme activity in % of maximum:
B | G | O | W | Y |
0-10 | 10-25 | 75-100 | 100 | 10-25 |
Product: | |
Cat. No. | Size |
E099S | 200 U |
E099 | 500 U |
E100 | 2500 U |