Ava II (Bme18 I, Eco47 I)

:Origin
Bacillus megaterium 18

:Concentration
5000 – 20000, u.a./ml

:Recognition site
G/GWCC
CCWG/G

:Reaction buffer
GM-buffer O, (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)

:Storage conditions
10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 µg/ml BSA; 50% glycerol

Storage temperature: -20°C

:Ligation
After 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.

:Non-specific hydrolysis
No nonspecific activity was detected after incubation of 1 μg of DNA with 20 u.a. of enzyme for 16 hours at 37°C.

Optimum temperature: 37°C

Inactivation 20 minutes: under 65°C

:Enzyme activity in % of maximum

BGOWY
10-2525-5010075-10010-25
Product
Cat. No.Size
E029S400 U
E0291000 U
E0305000 U