Origin:
Bacillus species 1720

Concentration:
10000, u.a./ml

Recognition site:
GC/TNAGC
CGANT/CG

Reaction buffer:
GM-buffer G , (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl₂ ; 50 mM NaCl; 1 mM DTT .)

Storage conditions:
10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol

Storage temperature:
-20°C

Ligation:
After 10-fold overdigestion with enzyme more than 80% of the DNA fragments can be ligated. Of these about 95% can be recut.

Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 10 u.a. of enzyme for 16 hours at 37°C.

Optimum temperature:
37°C

Inactivation 20 minutes:
under 80 °C

Enzyme activity in % of maximum:

B	G	O	W	Y
50-75	100	50-75	50-75	75-100

Product：
Cat. No.	Size
E185S	100 U
E185	200 U
E186	1000 U