:Origin
E.coli strain that carries the cloned EcoR V gene from Escherichia coli

:Concentration
20000, u.a./ml

:Recognition site
GAT/ATC
CTA/TAG

:Reaction buffer
GM-buffer W + BSA, (10mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl₂; 100 mM NaCl; 1 mM DTT + 100 μg/ml BSA.)

:Storage conditions
10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 µg/ml BSA; 50% glycerol.

:Storage temperature
-20°C

:Ligation
After 20-fold overdigestion with enzyme more than 90% of DNA fragments can be ligated and recut.

Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 20 u.a. of enzyme for 16 hours at 37°C.

:Optimum temperature
37°C

:Inactivation 20 minutes
under 80°C

:Enzyme activity in % of maximum

B	G	O	W	Y
0-10	25-50	50-75	100	25-50

Product：
Cat. No.	Size
E059S	1000 U
E059	2000 U
E060	10000 U