:Origin
Isolated from an E.coli strain that carries the cloned Kpn I gene from Klebsiella pneumonia

:Concentration
20000, u.a./ml

:Recognition site
GGTAC/C
C/CATGG

:Reaction buffer
GM-buffer B + BSA, (10mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl₂; 1mM DTT + 100 μg/ml BSA.)

:Storage conditions
10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1mM EDTA; 1 mM DTT; 200 µg/ml BSA; 50% glycerol.

:Storage temperature
-20°C

:Ligation
After 20-fold overdigestion with enzyme more than 90% of DNA fragments can be ligated and recut.

:Non-specific hydrolysis
No nonspecific activity was detected after incubation of 1 μg of DNA with 20 u.a. of enzyme for 16 hours at 37°C.

:Optimum temperature
37°C

:Inactivation 20 minutes
under 80°C

:Enzyme activity in % of maximum

B	G	O	W	Y
100	25-50	25-50	25-50	75-100

Product：
Cat. No.	Size
E079S	1000 U
E079	2000 U
E080	10000 U