:Origin
E.coli strain that carries the cloned EcoR V gene from Escherichia coli
:Concentration
20000, u.a./ml
:Recognition site
GAT/ATC
CTA/TAG
:Reaction buffer
GM-buffer W + BSA, (10mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT + 100 μg/ml BSA.)
:Storage conditions
10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 µg/ml BSA; 50% glycerol.
:Storage temperature
-20°C
:Ligation
After 20-fold overdigestion with enzyme more than 90% of DNA fragments can be ligated and recut.
Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 20 u.a. of enzyme for 16 hours at 37°C.
:Optimum temperature
37°C
:Inactivation 20 minutes
under 80°C
:Enzyme activity in % of maximum
B | G | O | W | Y |
0-10 | 25-50 | 50-75 | 100 | 25-50 |
Product: | |
Cat. No. | Size |
E059S | 1000 U |
E059 | 2000 U |
E060 | 10000 U |