:Origin
E.coli strain that carries the cloned Hae IIIgene from Haemophilus aegyptius
:Concentration
10000, u.a./ml
:Recognition site
GG/CC
CC/GG
:Reaction buffer
GM-buffer G, (10mM Tris-HCl(pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.)
:Storage conditions
10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1mM EDTA; 1 Mm DTT; 200 µg/ml BSA; 50% glycerol.
:Storage temperature
-20°C
:Ligation
After 20-fold overdigestion with enzyme more than 90% of DNA fragments can be ligated and recut.
:Non-specific hydrolysis
No nonspecific activity was detected after incubation of 1 μg of DNA with 20 u.a. of enzyme for 16 hours at 37°C.
:Optimum temperature
37°C
:Inactivation 20 minutes
under 80°C
:Enzyme activity in % of maximum
B | G | O | W | Y |
75-100 | 100 | 25-50 | 50-75 | 50-75 |
Product: | |
Cat. No. | Size |
E067S | 2000U |
E067 | 5000 U |
E068 | 25000 U |