:Origin
E.coli strain that carries the cloned Hae IIIgene from Haemophilus aegyptius

:Concentration
10000, u.a./ml

:Recognition site
GG/CC
CC/GG

:Reaction buffer
GM-buffer G, (10mM Tris-HCl(pH 7.6 at 25°C); 10 mM MgCl₂; 50 mM NaCl; 1 mM DTT.)

:Storage conditions
10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1mM EDTA; 1 Mm DTT; 200 µg/ml BSA; 50% glycerol.

:Storage temperature
-20°C

:Ligation
After 20-fold overdigestion with enzyme more than 90% of DNA fragments can be ligated and recut.

:Non-specific hydrolysis
No nonspecific activity was detected after incubation of 1 μg of DNA with 20 u.a. of enzyme for 16 hours at 37°C.

:Optimum temperature
37°C

:Inactivation 20 minutes
under 80°C

:Enzyme activity in % of maximum

B	G	O	W	Y
75-100	100	25-50	50-75	50-75

Product：
Cat. No.	Size
E067S	2000U
E067	5000 U
E068	25000 U