Hga I (BsuR I, Pho I)

:Origin
E. coli strain that carries the cloned Hga I gene from Haemophilus gallinarum

:Concentration
1000, u.a./ml

:Recognition site
GACGC(N)5/
CTGCG(N)10/

:Reaction buffer
GM-buffer B, (10mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.)

:Storage conditions
10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1mM EDTA; 1 mM DTT; 200 µg/ml BSA; 50% glycerol.

:Storage temperature
-20°C

:Ligation
After 3-fold overdigestion with enzyme more than 90% of DNA fragments can be ligated and recut.

:Non-specific hydrolysis
Incubation with more than 2 u.a. of enzyme per 1 μg of DNA and digestion > 1 hour are not recommended.

:Optimum temperature
37°C

:Inactivation 20 minutes
under 65°C

:Enzyme activity in % of maximum

BGOWY
10075-10010-2525-5050-75
Product
Cat. No.Size
E46150 U
E462250 U