:Origin
E. coli strain that carries the cloned Hga I gene from Haemophilus gallinarum
:Concentration
1000, u.a./ml
:Recognition site
GACGC(N)5/
CTGCG(N)10/
:Reaction buffer
GM-buffer B, (10mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.)
:Storage conditions
10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1mM EDTA; 1 mM DTT; 200 µg/ml BSA; 50% glycerol.
:Storage temperature
-20°C
:Ligation
After 3-fold overdigestion with enzyme more than 90% of DNA fragments can be ligated and recut.
:Non-specific hydrolysis
Incubation with more than 2 u.a. of enzyme per 1 μg of DNA and digestion > 1 hour are not recommended.
:Optimum temperature
37°C
:Inactivation 20 minutes
under 65°C
:Enzyme activity in % of maximum
B | G | O | W | Y |
100 | 75-100 | 10-25 | 25-50 | 50-75 |
Product: | |
Cat. No. | Size |
E461 | 50 U |
E462 | 250 U |