Origin:
Micrococcus roseus X

Concentration:
5000 – 15000, u.a./ml

Recognition site:
GAANN/NNTTC
CTTNN/NNAAG

Reaction buffer:
GM-buffer W, (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl₂; 100 mM NaCl; 1 mM DTT.)

Storage conditions:
10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol.

Storage temperature:
-20°C

Ligation:
After 5-fold overdigestion with enzyme more than 50% of DNA fragments can be ligated and recut.

Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 10 u.a. of enzyme for 16 hours at 37°C.

Optimum temperature:
37°C

Inactivation 20 minutes:
under 65°C

Enzyme activity in % of maximum:

B	G	O	W	Y
50-75	50-75	50-75	100	25-50

Product：
Cat. No.	Size
E249S	100 U
E249	500 U
E250	2500 U