Origin:
Nocardia rubra

Concentration:
5000 – 10000, u.a./ml

Recognition site:
TCG/CGA
AGC/GCT

Reaction buffer:
GM-buffer W, (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl₂; 100 mM NaCl; 1 mM DTT.)

Storage conditions:
10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol.

Storage temperature:
-20°C

Ligation:
After 5-fold overdigestion with enzyme about 20% of DNA fragments can be ligated. In the presence of 10% PEG ligation is better.

Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 10 u.a. of enzyme for 16 hours at 37°C.

Optimum temperature:
37°C

Inactivation 20 minutes:
under 80°C

Enzyme activity in % of maximum:

B	G	O	W	Y
0-10	10-25	75-100	100	10-25

Product：
Cat. No.	Size
E099S	200 U
E099	500 U
E100	2500 U