Origin:
Bacillus stearothermophillus MB

Concentration:
5000 – 10000, u.a./ml

Recognition site:
/GATC
CTAG/

Reaction buffer:
GM-buffer O, (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl₂; 100 mM NaCl; 1 mM DTT.)

Storage conditions:
10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 200 µg/ml BSA.

Storage temperature:
-20°C

Ligation:
After 5-fold overdigestion with enzyme more than 95% of DNA fragments can be ligated and recut.

Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 20 u.a. of enzyme for 16 hours at 65°C.

Optimum temperature:
65°C

Inactivation 20 minutes:
under 80°C

Enzyme activity in % of maximum:

B	G	O	W	Y
10-25	25-50	100	75-100	10-25

Product：
Cat. No.	Size
E119	200 U
E120	1000 U