Origin:
Pseudomonas pseudoalcaligenes

Concentration:
500 – 1000, u.a./ml

Recognition site:
GAGTC(N)₄/
CTCAG(N)₅/

Reaction buffer:
GM-buffer Y + BSA, (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT + 100 μg/ml BSA.)

Storage conditions:
10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol.

Storage temperature:
-20°C

Ligation:
After 2-fold overdigestion with enzyme 20% of DNA fragments can be ligated and recut.

Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 2 u.a. of enzyme for 16 hours at 37°C.

Optimum temperature:
37°C

Inactivation 20 minutes:
under 65°C

Enzyme activity in % of maximum:

B	G	O	W	Y
50-75	10-25	0-10	25-50	100

Product：
Cat. No.	Size
E269	25 U
E270	125 U