Origin:
Pseudomonas pseudoalcaligenes
Concentration:
500 – 1000, u.a./ml
Recognition site:
GAGTC(N)4/
CTCAG(N)5/
Reaction buffer:
GM-buffer Y + BSA, (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT + 100 μg/ml BSA.)
Storage conditions:
10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol.
Storage temperature:
-20°C
Ligation:
After 2-fold overdigestion with enzyme 20% of DNA fragments can be ligated and recut.
Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 2 u.a. of enzyme for 16 hours at 37°C.
Optimum temperature:
37°C
Inactivation 20 minutes:
under 65°C
Enzyme activity in % of maximum:
B | G | O | W | Y |
50-75 | 10-25 | 0-10 | 25-50 | 100 |
Product: | |
Cat. No. | Size |
E269 | 25 U |
E270 | 125 U |