:Origin
E.coli strain that carries the cloned EcoR I gene from Escherichia coli

:Concentration
20000, u.a./ml

:Recognition site
G/AATTC
CTTAA/G

:Reaction buffer
GM-buffer EcoR I + BSA, (100mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl₂; 50 mM NaCl; 1 mM DTT + 100 μg/ml BSA.)

:Storage conditions
10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol.

:Storage temperature
-20°C

:Ligation
After 40-fold overdigestion with enzyme more than 95% of DNA fragments can be ligated and recut.

:Non-specific hydrolysis
No nonspecific activity was detected after incubation of 1 μg of DNA with 40 u.a. of enzyme for 16 hours at 37°C.

:Optimum temperature
37°C

:Inactivation 20 minutes
under 65°C

:Enzyme activity in % of maximum

B	G	O	W	Y
50-75	75-100	75-100	75-100	50-75

Product：
Cat. No.	Size
E057S	5000 U
E057	10000 U
E058	50000 U