Origin:
Bacillus stearothermophilus MW
Concentration:
2000 – 10000, u.a./ml
Recognition site:
GCNNNNN/NNGC
CGNN/NNNNNCG
Reaction buffer:
GM-buffer Y, (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Storage conditions:
10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol.
Storage temperature:
-20°C; For long term stroage ( > 7 days), store at -70°C.
Ligation:
After 5-fold overdigestion with enzyme more than 95% of DNA fragments can be ligated and recut.
Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 10 u.a. of enzyme for 16 hours at 55°C.
Optimum temperature:
55°C
Inactivation 20 minutes:
under 80°C
Enzyme activity in % of maximum:
B | G | O | W | Y |
10-25 | 25-50 | 25-50 | 50-75 | 100 |
Product: | |
Cat. No. | Size |
E459S | 100 U |
E459 | 500 U |
E460 | 2500 U |