Origin:
Bacillus stearothermophilus MW

Concentration:
2000 – 10000, u.a./ml

Recognition site:
GCNNNNN/NNGC
CGNN/NNNNNCG

Reaction buffer:
GM-buffer Y, (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)

Storage conditions:
10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol.

Storage temperature:
-20°C; For long term stroage ( > 7 days), store at -70°C.

Ligation:
After 5-fold overdigestion with enzyme more than 95% of DNA fragments can be ligated and recut.

Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 10 u.a. of enzyme for 16 hours at 55°C.

Optimum temperature:
55°C

Inactivation 20 minutes:
under 80°C

Enzyme activity in % of maximum:

B	G	O	W	Y
10-25	25-50	25-50	50-75	100

Product：
Cat. No.	Size
E459S	100 U
E459	500 U
E460	2500 U