:Origin
Isolated from an E.coli strain that carries the cloned HinP1 I gene from Haemophilus species A1

:Concentration
20000, u.a./ml

:Recognition site
G/CGC
CGC/G

:Reaction buffer
GM-buffer Y, (33mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1mM DTT.)

:Storage conditions
10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol.

:Storage temperature
-20°C

:Ligation
After 20-fold overdigestion with enzyme more than 90% of DNA fragments can be ligated and recut.

:Non-specific hydrolysis
No nonspecific activity was detected after incubation of 1 μg of DNA with 20 u.a. of enzyme for 16 hours at 37°C.

:Optimum temperature

37°C

:Inactivation 20 minutes
under 80°C

:Enzyme activity in % of maximum

B	G	O	W	Y
50-75	50-75	25-50	25-50	100

Product：
Cat. No.	Size
E069S	500 U
E069	1000 U
E070	5000 U