:Origin Bacillus stearothermophilus DE :Concentration 10000 – 50000, u.a./ml :Recognition site C/TNAG GANT/C :Reaction buffer GM-buffer G, (10mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.) :Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol. :Storage temperature -20°C :Ligation After […]
:Origin Arthrobacter species LE3860 :Concentration 20000, u.a./ml :Recognition site GCG/C C/GCG :Reaction buffer GM-buffer O, (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) :Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol Storage temperature: -20°C […]
:Origin E.coli strain that carries BamH I gene from Bacillus amyloliquefaciens H :Concentration 20000 – 50000, u.a./ml :Recognition site G/GATCC CCTAG/G :Reaction buffer GM-buffer G + BSA, (10mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT + 100 μg/ml BSA.) :Storage conditions 10 mM Tris-HCL(pH 7.6); 50 mM NaCl; […]